Apr 10, 2013
An entirely automated method to score DSS-induced colitis in mice by digital image analysis of pathology slides
Kozlowski C, Jeet S, Beyer J, Guerrero S, Lesch J, Wang X, Devoss J, Diehl L , Dis Model Mech , DOI: 10.1242/dmm.011759
The DSS (dextran sulfate sodium) model of colitis is a mouse model of inflammatory bowel disease. Microscopic symptoms include loss of crypt cells from the gut lining and infiltration of inflammatory cells into the colon. An experienced pathologist requires several hours per study to score histological changes in selected regions...
Mar 22, 2013
Combat or Surveillance? Evaluation of the Heterogeneous Inflammatory Breast Cancer Microenvironment
Krüger JM, Wemmert C, Sternberger L, Bonnas C, Dietmann G, Gançarski P, Feuerhake F , J Pathol , DOI: 10.1002/path.4150
Evaluation of specific lymphocyte subsets is important in understanding the microenvironment in cancer and holds promise as prognostic parameter in invasive breast cancer. To address this we used digital image analysis to integrate cell abundance, distance metrics, neighbourhood relationships, and sample heterogeneity into comprehensive assessment of immune infiltrates. Lymphocyte and...
Mar 1, 2013
Longitudinal Expression Analysis of αv Integrins in Human Gliomas Reveals Upregulation of Integrin αvβ3 as a Negative Prognostic Factor
J. Schittenhelm, E. I. Schwab, J. Sperveslage, M. Tatagiba, R. Meyermann, F. Fend, S. L. Goodman, B. Sipos , Journal of Neuropathology & Experimental Neurology , DOI: 10.1097/NEN.0b013e3182851019
Integrin inhibitors targeting αv series integrins are being tested for their therapeutic potential in patients with brain tumors, but pathologic studies have been limited by lack of antibodies suitable for immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded specimens. We compared the expression of αv integrins by IHC in brain tumor and normal...
Feb 28, 2013
High Heregulin Expression Is Associated with Activated HER3 and May Define an Actionable Biomarker in Patients with Squamous Cell Carcinomas of the Head and Neck
D. S. Shames, J. Carbon, K. Walter, A. M. Jubb, C. Kozlowski, T. Januario, D. An, L. Fu, Y. Xiao, R. Raja, B. Jiang, A. Malekafzali, H. Stern, J. Settleman, T. R. Wilson, G. M. Hampton, R. L. Yauch, A. Pirzkall , L. C. Amler , PLoS ONE , DOI: 10.1371/journal.pone.0056765
Purpose: Tumors with oncogenic dependencies on the HER family of receptor tyrosine kinases (RTKs) often respond well to targeted inhibition. Our previous work suggested that many cell lines derived from squamous cell carcinomas of the head and neck (SCCHNs) depend on autocrine signaling driven by HER2/3 dimerization and high-level co-expression...
Feb 28, 2013
Vascular E-Selectin Expression Correlates with CD8 Lymphocyte Infiltration and Improved Outcome in Merkel Cell Carcinoma
O. K. Afanasiev, K. Nagase, W. Simonson, N. Vandeven, A. Blom, D. M. Koelle, R. Clark, P. Nghiem , Journal of Investigative Dermatology , DOI: 10.1038/jid.2013.36
Merkel cell carcinoma (MCC) is an aggressive, polyomavirus-linked skin cancer. Although CD8 lymphocyte infiltration into the tumor is strongly correlated with improved survival, these cells are absent or sparse in most MCCs. We investigated whether specific mechanisms of T-cell migration may be commonly disrupted in MCC tumors with poor CD8...