Mar 3, 2018
Fingolimod inhibits brain atrophy and promotes brain-derived neurotrophic factor in an animal model of multiple sclerosis
P. A. Smith, C. Schmid, S. Zurbruegg, M. Jivkov, A. Doelemeyer, D. Theil, V. Dubost, N. Beckmann , Journal of Neuroimmunology , DOI: 10.1016/j.jneuroim.2018.02.016
Longitudinal brain atrophy quantification is a critical efficacy measurement in multiple sclerosis (MS) clinical trials and the determination of No Evidence of Disease Activity (NEDA). Utilising fingolimod as a clinically validated therapy we evaluated the use of repeated brain tissue volume measures during chronic experimental autoimmune encephalomyelitis (EAE) as a...
Mar 2, 2018
Computationally-Guided Development of a Stromal Inflammation Histologic Biomarker in Lung Squamous Cell Carcinoma
D. Xia, R. Casanova, D. Machiraju, T. D. McKee, W. Weder, A. H. Beck, A. Soltermann , Scientific Reports , DOI: 10.1038/s41598-018-22254-4
The goal of this study is to use computational pathology to help guide the development of human-based prognostic H&E biomarker(s) suitable for research and potential clinical use in lung squamous cell carcinoma (SCC). We started with high-throughput computational image analysis with tissue microarrays (TMAs) to screen for histologic features associated...
Feb 23, 2018
Hu antigen R (HuR) heterogeneous expression quantification as a prognostic marker of melanoma
N. Liaudet, M. Fernandez, L. Fontao, G. Kaya, R. Merat , Journal of Cutaneous Pathology , DOI: 10.1111/cup.13119
Background: Prognostic markers for melanoma, particularly for stage II disease, are needed for the risk-benefit evaluation of future adjuvant therapies. The mainly nuclear RNA-binding protein human antigen R (HuR) regulates the protein expression of thousands of mRNAs, its own heterogeneous expression could therefore reflect tumor heterogeneity and plasticity. Here, we...
Feb 20, 2018
Elucidating the Burden of HIV in Tissues Using Multiplexed Immunofluorescence and In Situ Hybridization: Methods for the Single-Cell Phenotypic Characterization of Cells Harboring HIV In Situ
J. J. Vasquez, R. Hussien, B. Aguilar-Rodriguez, H. Junger, D. Dobi, T. J. Henrich, C. Thanh, E. Gibson, L. E. Hogan, J. McCune, P. W. Hunt, C. A. Stoddart, Z. G. Laszik , Journal of Histochemistry & Cytochemistry , DOI: 10.1369/0022155418756848
Persistent tissue reservoirs of HIV present a major barrier to cure. Defining subsets of infected cells in tissues is a major focus of HIV cure research. Herein, we describe a novel multiplexed in situ hybridization (ISH) (RNAscope) protocol to detect HIV-DNA (vDNA) and HIV-RNA (vRNA) in formalin-fixed paraffin-embedded (FFPE) human...
Feb 18, 2018
Presence of TERT Promoter Mutations is a Secondary Event and Associates with Elongated Telomere Length in Myxoid Liposarcomas
M. S. Ventura Ferreira, M. Crysandt, T. Braunschweig, E. Jost, B. Voss, A.-S. Bouillon, R. Knuechel, T. H. Brümmendorf, F. Beier , International Journal of Molecular Sciences , DOI:10.3390/ijms19020608
The occurrence of TERT promoter mutations has been well described in soft tissue sarcomas (STS). However, the biological role of these mutations as well as their impact on telomere length in STS is still unclear. We analyzed 116 patient samples diagnosed with 22 distinct histological subtypes of bone and STS...