Nov 17, 2014
Imaging and Analysis of RNA Biomarkers In Situ via RNAscope and SpotStudio
Dr. Xiao-Jun Ma, Chief Scientific Officer, Advanced Cell Diagnostics, Hayward, California, USA
RNA in situ hybridization, ISH, is a powerful tool for establishing spatial and cell type-specific gene expression in tissue. Traditional RNA ISH methods are technically challenging to perform and lack sufficient sensitivity and specificity for most clinically relevant biomarkers. RNAscope is a novel RNA ISH technology capable of detecting RNA in situ with single molecule sensitivity and single cell resolution and is compatible with routine formalin-fixed paraffin-embedded clinical specimen. The single-molecule detection capability of RNAscope in particular enables straightforward quantification of RNA in single cells by counting signal dots. We have developed a dedicated image analysis software application =RNAscope SpotStudio= using Definiens’ Cognition Network Technology for quantitative analysis of RNAscope-stained slides. I will discuss how RNAscope and image analysis can be used together in cancer biomarker studies.